
Instrumentation
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Carrier Gas
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Injection Site
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Column and Oven
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Detector
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Data System

STATIONARY PHASE
CARRIER GAS
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Carrier Gases are inert and unreactive.
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Common gases are; He, H2, N2 and even Ar.
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Helium is the most commonly used gas as it can yield equal or even better results than Hyrdogen when flow rates are optomized
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Hydrogen yields the fastest separation rates however it is incredibly explosive and flammable.
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These gasses must be pure, no impurities what so ever.
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Stationary Phases are usually liquids but can sometimes be a very thin micro layer of solid.
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Solids are usually not used due to the large "tailing" issue they have one the chromatogram.
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"Like" disolves "Like"
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This applied to liquid stationary phases.
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Nonpolar columns are best for nonpolar solutes, polar columns for polar solutes, ect.
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Arylene
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Good choice for a liquid stationary phase.
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Can withstand high temperatures and hold their stability.
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Resistant to bleeding at high temperatures.
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DISTRIBUTION
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Measures tendency of analyte to be attracted to stationary phase
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Kc = [Cs]/[Cm]
Cs = concentration of analyte in stationary phase
Cm = concentration of analyte in mobile phase
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FORMULA
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Resolution
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Plate Height
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Theorectical Plate Number
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Retention Index



