top of page

Instrumentation

  • Carrier Gas

  • Injection Site

  • Column and Oven

  • Detector

  • Data System

STATIONARY PHASE

CARRIER GAS

  • Carrier Gases are inert and unreactive.

  • Common gases are; He, H2, N2 and even Ar. 

  • Helium is the most commonly used gas as it can yield equal or even better results than Hyrdogen when flow rates are optomized

    • Hydrogen yields the fastest separation rates however it is incredibly explosive and flammable. 

  • These gasses must be pure, no impurities what so ever. 

  • Stationary Phases are usually liquids but can sometimes be a very thin micro layer of solid.

    • Solids are usually not used due to the large "tailing" issue they have one the chromatogram.

  • "Like" disolves "Like"

    • This applied to liquid stationary phases. 

    • Nonpolar columns are best for nonpolar solutes, polar columns for polar solutes, ect. 

  • Arylene

    • Good choice for a liquid stationary phase. 

    • Can withstand high temperatures and hold their stability.

    • Resistant to bleeding at high temperatures. 

DISTRIBUTION

  • Measures tendency of analyte to be attracted to stationary phase

    • Kc = [Cs]/[Cm]

       

      Cs = concentration of analyte in stationary phase

      Cm = concentration of analyte in mobile phase

FORMULA

  • Resolution

 

 

  • Plate Height

 

 

  • Theorectical Plate Number

 

 

  • Retention Index 

Analytical Chemistry

Hartwitck College

© 2015 Alicia & Winston

bottom of page