Factors and More!
There are a few factors that will influence the separation output of gas chromatography.
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Boiling point
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Lower boiling point results in shorter retention time
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Polarity
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If stationary phase and compound have same polarity, the retention time of the compound will increased
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Column temperature
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Extremely high temperatures results in short retention times
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There are also quite a few advantages to using GC
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Fast analysis of separations
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Manipulation of temperature and stationary phase chemistry
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Accurate for quantitative analysis
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Highly efficient and high resolution
However there are a few disadvantages to the method
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Limited to volatile samples
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Not suitable for thermodynamically unstable samples at high temperatures
APPLICATIONS
Pharmaceutical Applications
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GC is a highly saught out method of analysis in Pharmaceutical analysis. Because of it's ability to separate components and even test the purities of the samples and do so in a quick manner with use of a very small amount of sample it's the prefered method. It's very sensative and incredible accurate so for the sake of time, money and sample it's well saught out.
Food and Drink Applications
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Typically, GC is useful for analyzing nonpolar and semi-polar, volatile and semi-volatile chemicals. Without chemical derivatization, GC is often used for the analysis of sterols, oils, low chain fatty acids, aroma components and off- flavors, and many contaminants, such as pesticides, industrial pollutants, and certain types of drugs in foods.
Evironmental Applications
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Gas chromatography has become an important analytical tool in virtually every phase of the petroleum industry, from exploration of crude oil and refining of finished products to research on new petrochemicals. Because of the variety and complexity of sample types, petroleum chemists use a broad spectrum of gas chromatographic methods.